Open AccessSequence analysis of the gtfB gene from Streptococcus mutans
Author(s) -
Teruaki Shiroza,
Shunsaku Ueda,
Howard K. Kuramitsu
Publication year - 1987
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.169.9.4263-4270.1987
Subject(s) - biology , glucosyltransferase , open reading frame , gene , nucleic acid sequence , streptococcus mutans , peptide sequence , coding region , sequence analysis , genetics , microbiology and biotechnology , biochemistry , bacteria
The nucleotide sequence of the gtfB gene from Streptococcus mutans GS-5, coding for glucosyltransferase I activity, was determined. The gene codes for a strongly hydrophilic protein with a molecular size of 165,800 daltons. The deduced amino acid sequence revealed a typical gram-positive bacterial signal sequence at the NH2 terminus of the protein and 3.5 direct repeating units (each containing 65 amino acids) at the COOH terminus. Nucleotide sequencing of the region immediately downstream from the gtfB gene revealed the presence of a putative gene coding for an extracellular protein. This open reading frame is partially homologous to the gtfB gene.