Open AccessoriT sequence of the antibiotic resistance plasmid R100
Author(s) -
Sarah A. McIntire,
Walter B. Dempsey
Publication year - 1987
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.169.8.3829-3832.1987
Subject(s) - biology , plasmid , homology (biology) , genetics , nucleic acid sequence , sequence homology , dna , nucleotide , conserved sequence , sequence (biology) , microbiology and biotechnology , gene , base sequence
We present the nucleotide sequence of the oriT region from plasmid R100. Comparison to other IncF plasmids revealed homology around the proposed nick sites as well as conservation of inverted repeated sequences in the nonhomologous region. Three areas showed strong homology (eight of nine nucleotides) to the consensus sequence for binding of integration host factor, suggesting a role for this DNA-binding protein in nicking at oriT.