Open AccessIn vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae
Author(s) -
Po Keung Wong,
David L. Popham,
John Keener,
Sydney Kustu
Publication year - 1987
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.169.6.2876-2880.1987
Subject(s) - biology , operon , rna polymerase , sigma factor , transcription (linguistics) , klebsiella pneumoniae , promoter , microbiology and biotechnology , dna , polymerase , biochemistry , gene , rna , gene expression , escherichia coli , linguistics , philosophy
In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K. pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an alternate sigma factor; NTRC, an auxiliary DNA-binding protein; and NTRB, a bifunctional enzyme that controls the activity of NTRC by covalent modification (A.J. Ninfa and B. Magasanik, Proc. Natl. Acad. Sci. USA 83:5909, 1986). Two DNA-binding sites for NTRC lie approximately 150 base pairs upstream of the nifLA promoter.