Open AccessIdentification, mapping, and cloning of the gene encoding cyanase in Escherichia coli K-12
Author(s) -
Young-Chul Sung,
Dawn A. Parsell,
Paul N. Anderson,
Jamesa . Fuchs
Publication year - 1987
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.169.6.2639-2642.1987
Subject(s) - bglii , biology , escherichia coli , operon , plasmid , pbr322 , gene , structural gene , microbiology and biotechnology , cloning (programming) , molecular cloning , genetics , ecorv , complementary dna , ecori , computer science , programming language
The gene in Escherichia coli for cyanase, designated cynS, was localized to a BglII restriction site approximately 1.7 kilobases from the lacA end of the lac operon. The gene was cloned into the pUC13 vector. Maxicell analysis of plasmid-encoded proteins confirmed that the BglII site is in the region encoding the structural gene for cyanase. Cyanase-deficient strains had increased sensitivity to cyanate and were not able to use cyanate as a nitrogen source.