Open AccessTranscription patterns of Rhizobium meliloti symbiotic plasmid pSym: identification of nifA-independent fix genes
Author(s) -
M. David,
Odile Domergue,
P. Pogc,
Daniel Kahn
Publication year - 1987
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.169.5.2239-2244.1987
Subject(s) - biology , plasmid , gene , locus (genetics) , genetics , rhizobium , transcription (linguistics) , gene cluster , nitrogen fixation , gene product , gene expression , bacteria , linguistics , philosophy
We performed a systematic survey of transcription of a large region of the Rhizobium meliloti symbiotic plasmid pSym. This led to the discovery of two new sequences induced during symbiosis. The first sequence was linked to the known nitrogen fixation (nif-fix) gene cluster, and its expression depended on the nifA gene product. The second sequence was a novel fix locus (M.-H. Renalier, J. Batut, J. Ghai, B. Terzaghi, M. Gherardi, M. David, A.-M. Garnerone, J. Vasse, G. Truchet, T. Huguet, and P. Boistard, J. Bacteriol. 169:2231-2238, 1987) whose expression was independent of the nifA gene product; therefore this fix locus undergoes a novel type of symbiotic regulation.