Open AccessIron-molybdenum cofactor synthesis in Azotobacter vinelandii Nif- mutants
Author(s) -
Juan Imperial,
Vinod K. Shah,
Rodolfo A. Ugalde,
Paul W. Ludden,
Winston J. Brill
Publication year - 1987
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.169.4.1784-1786.1987
Subject(s) - azotobacter vinelandii , mutant , molybdenum cofactor , biology , cofactor , biochemistry , molybdenum , nitrogenase , chemistry , bacteria , enzyme , gene , genetics , nitrogen fixation , inorganic chemistry
Nif- mutants of Azotobacter vinelandii defective in dinitrogenase activity synthesized iron-molybdenum cofactor (FeMo-co) and accumulated it in two protein-bound forms: inactive dinitrogenase and a possible intermediate involved in the FeMo-co biosynthetic pathway. FeMo-co from both these proteins could activate apo-dinitrogenase from FeMo-co-deficient mutants.