Open Access2',3'-Dialdehyde of ATP: a specific, irreversible inhibitor of component A3 of the methylreductase system of Methanobacterium thermoautotrophicum
Author(s) -
Pierre E. Rouvière,
R. S. Wolfe
Publication year - 1987
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.169.4.1737-1739.1987
Subject(s) - methanobacterium , biology , biochemistry , stereochemistry , atp synthase , adenosine triphosphate , purine , thio , cofactor , enzyme , chemistry , gene , archaea
Among 17 purine and ATP derivatives tested, 3 were found to totally inhibit the methyl coenzyme M methylreductase system of Methanobacterium thermoautotrophicum at a concentration of 5 mM; these derivatives were 8-azido-ATP, alpha, beta-thio-ADP and 2',3'-dialdehyde-ATP. 2',3'-Dialdehyde-ATP specifically and irreversibly bound to component A3 of the methylreductase system during ATP activation of the system.