Open AccessEffect of decoyinine on the regulation of alpha-amylase synthesis in Bacillus subtilis
Author(s) -
Wayne L. Nicholson,
Glenn H. Chambliss
Publication year - 1987
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.169.12.5867-5869.1987
Subject(s) - catabolite repression , bacillus subtilis , biology , alpha amylase , mutant , psychological repression , strain (injury) , biochemistry , enzyme repression , fed batch culture , amylase , alpha (finance) , enzyme , microbiology and biotechnology , bacteria , genetics , gene , gene expression , fermentation , medicine , construct validity , nursing , anatomy , patient satisfaction
Decoyinine, an inhibitor of GMP synthetase, allows sporulation in Bacillus subtilis to initiate and proceed under otherwise catabolite-repressing conditions. The effect of decoyinine on alpha-amylase synthesis in B. subtilis, an event which exhibits regulatory features resembling sporulation initiation, was examined. Decoyinine did not overcome catabolite repression of alpha-amylase synthesis in a wild-type strain of B. subtilis but did cause premature and enhanced synthesis in a mutant strain specifically blocked in catabolite repression of alpha-amylase synthesis. Decoyinine had no effect on alpha-amylase enzymatic activity. Thus, it appears that the catabolite control mechanisms governing alpha-amylase synthesis and sporulation in B. subtilis differ in their responses to decoyinine and hence must consist at least partially of separate components.