Open AccessExpression of the bacteriophage T4 denV structural gene in Escherichia coli
Author(s) -
Adrián Recinos,
Mary Lou Augustine,
Kay M. Higgins,
R. Stephen Lloyd
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.168.2.1014-1018.1986
Subject(s) - biology , escherichia coli , bacteriophage , plasmid , gene , microbiology and biotechnology , lambda phage , structural gene , gene product , dna , gene expression , genetics
The expression of the T4 denV gene, which previously had been cloned in plasmid constructs downstream of the bacteriophage lambda hybrid promoter-operator oLpR, was analyzed under a variety of growth parameters. Expression of the denV gene product, endonuclease V, was confirmed in DNA repair-deficient Escherichia coli (uvrA recA) by Western blot analyses and by enhancements of resistance to UV irradiation.