Open AccessCloning and characterization of the cysAMK region of Salmonella typhimurium
Author(s) -
Magdalena Hulanicka,
Christine Garrett,
Grażyna Jagura-Burdzy,
Nicholas M. Kredich
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.168.1.322-327.1986
Subject(s) - biology , pbr322 , genetics , cloning (programming) , restriction map , dna , gene , southern blot , molecular cloning , microbiology and biotechnology , lysogen , lambda phage , escherichia coli , plasmid , bacteriophage , gene expression , computer science , programming language
A total of 30 kilobases of DNA comprising the cysAMK region of S. typhimurium was cloned as a series of fragments in phage lambda 1059. The genetic organization of this region was established through studies of gene expression from fragments subcloned in pBR322 and from blot hydridization analyses of restriction sites in chromosomal DNA from multisite deletion strains. The results give a gene order of cysA-cysM-crr-ptsl-ptsH-cysK over a distance of approximately 12 kilobases. cysM and cysA have been cloned and expressed in pBR322; attempts to obtain stable pBR322 derivatives carrying cysK were unsuccessful.