Open AccessGenetics of NAD metabolism in Salmonella typhimurium and cloning of the nadA and pnuC loci
Author(s) -
Simin Tirgari,
Michael P. Spector,
John W. Foster
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.167.3.1086-1088.1986
Subject(s) - biology , chloramphenicol , salmonella , genetics , cloning (programming) , gene , microbiology and biotechnology , gel electrophoresis , escherichia coli , nad+ kinase , bacteria , biochemistry , enzyme , computer science , programming language
The nadA and pnuC loci of S. typhimurium were cloned and found to reside within a 2.2-kilobase region. Two-dimensional O'Farrell gel electrophoresis of the proteins produced after chloramphenicol amplification and subsequent release from chloramphenicol inhibition revealed NadA and PnuC to be 43,000- and 25,000-molecular-weight proteins, respectively. The data indicated that nadA and pnuC represent two distinct genes.