Transposition of Tn917 in Bacillus megaterium | Zendy

N A Bohall | Zendy; Patricia S. Vary | Zendy

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Transposition of Tn917 in Bacillus megaterium

Author(s) -

N A Bohall,

Patricia S. Vary

Publication year - 1986

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.167.2.716-718.1986

Subject(s) - biology , bacillus megaterium , transposable element , transposition (logic) , auxotrophy , reversion , genetics , plasmid , transduction (biophysics) , microbiology and biotechnology , gene , mutant , botany , bacteria , phenotype , linguistics , philosophy

Transposon Tn917, carried on plasmid pTV1, was introduced into Bacillus megaterium and transposed efficiently and apparently randomly. Insertional mutations included at least eight different auxotrophic loci, two carbon source loci, and sporulation loci. One trp::Tn917 mutation was further verified as an insertion by both reversion and transduction.

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