Open AccessTransposition of the gram-positive transposon Tn917 in Escherichia coli
Author(s) -
Howard K. Kuramitsu,
M J Casadaban
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.167.2.711-712.1986
Subject(s) - transposable element , biology , transposition (logic) , plasmid , escherichia coli , bacillus subtilis , tn3 transposon , microbiology and biotechnology , transposase , genetics , bacteria , dna , gene , genome , linguistics , philosophy
The streptococcal transposon Tn917 was demonstrated to transpose in Escherichia coli from the Bacillus subtilis-E. coli shuttle plasmid pHK1207 into an F' plasmid derivative. Subsequently, a second round of transposition from the F' plasmid into pACYC184 could be readily demonstrated. These results represent the initial demonstration of the transposition of a gram-positive transposon in a gram-negative bacterium at a relatively high frequency.
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