Open AccessMapping of mutations in Pseudomonas aeruginosa defective in pyoverdin production
Author(s) -
Robert G. Ankenbauer,
Larry F. Hanne,
C D Cox
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.167.1.7-11.1986
Subject(s) - biology , mutant , pseudomonas aeruginosa , transposon mutagenesis , transposable element , pseudomonadales , pseudomonadaceae , mutagenesis , mutation , escherichia coli , pyoverdine , microbiology and biotechnology , biochemistry , bacteria , genetics , gene
Twelve mutants of Pseudomonas aeruginosa PAO defective in pyoverdin production were isolated (after chemical and transposon mutagenesis) that were nonfluorescent and unable to grow on medium containing 400 microM ethylenediaminedi(o-hydroxyphenylacetic acid). Four mutants were unable to produce hydroxamate, six were hydroxamate positive, one was temperature sensitive for pyoverdin production, and another was unable to synthesize pyoverdin on succinate minimal medium but was capable of synthesizing pyoverdin when grown on Casamino Acids medium (Difco Laboratories, Detroit, Mich.). The mutations were mapped on the PAO chromosome. All the mutations affecting pyoverdin production were located at 65 to 70 min, between catA1 and mtu-9002.