Open AccessRegulatory mutation that controls nif expression and histidine transport in Azospirillum brasilense
Author(s) -
M. Fischer,
Efrat Levy,
Tania Geller
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.167.1.423-426.1986
Subject(s) - azospirillum brasilense , biology , histidine , mutant , mutagenesis , mutation , biochemistry , nitrogenase , microbiology and biotechnology , enzyme , bacteria , genetics , nitrogen fixation , gene
Mutagenesis of Azospirillum brasilense with nitrosoguanidine and selection on ethylenediamine yielded prototrophs which fixed nitrogen in the presence of ammonia. Nitrogenase activity in mutant strains exceeded that of the wild type three- to sixfold. The same mutants were also constitutive for histidine transport. Enzyme activities involved in ammonia assimilation were not affected by the mutation. The data suggest that the mutation occurred at a site which regulates nif and histidine transport functions.