Open AccessRoles of H+-ATPase and proton motive force in ATP-dependent protein translocation in vitro
Author(s) -
L L Chen,
Phang C. Tai
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.167.1.389-392.1986
Subject(s) - biology , chemiosmosis , chromosomal translocation , operon , atpase , mutant , escherichia coli , microbiology and biotechnology , transport protein , atp synthase , adenosine triphosphate , biochemistry , biophysics , enzyme , gene
Membrane vesicles from an Escherichia coli mutant with a deletion of the uncBC operon required ATP to translocate proteins, thus ruling out an essential role of F1F0-H+-ATPase in ATP-dependent protein translocation. Moreover, proteins could be translocated in the absence of proton motive force. At suboptimal ATP concentrations, D-lactate stimulated protein translocation, indicating that proton motive force, although insufficient to support translocation, could facilitate the process.