Open AccesscamR, a negative regulator locus of the cytochrome P-450cam hydroxylase operon
Author(s) -
Hiroaki Koga,
Hironori Aramaki,
Eiichi Yamaguchi,
Kenji Takeuchi,
Tadao Horiuchi,
I. C. Gunsalus
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.166.3.1089-1095.1986
Subject(s) - biology , operon , pseudomonas putida , regulator , plasmid , cytochrome , microbiology and biotechnology , gene , genetics , biochemistry , enzyme , escherichia coli
A 4.27-kilobase insert from a HindIII DNA library of Pseudomonas putida carrying the CAM plasmid allowed coordinate expression of genes camD and camC under control of camR, an upstream regulator. The camC gene specifies cytochrome P-450cam, and camD specifies the 5-exo-alcohol dehydrogenase. A 1.38-kilobase deletion from the insert results in the constitutive expression of genes camC and camD; transformation in trans restores the substrate control, indicating that camR is a negative regulator.