Open AccessPolyadenylated RNA isolated from the archaebacterium Halobacterium halobium
Author(s) -
J W Brown,
John N. Reeve
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.166.2.686-688.1986
Subject(s) - biology , rna , halobacteriaceae , polyadenylation , nucleotide , halobacterium , methanococcus , biochemistry , halobacterium salinarum , halophile , bacteria , microbiology and biotechnology , archaea , genetics , gene
Polyadenylated [poly(A)+] RNA has been isolated from the halophilic archaebacterium Halobacterium halobium by binding, at 4 degrees C, to oligo(dT)-cellulose. H. halobium contains approximately 12 times more poly(A) per unit of RNA than does the methanogenic archaebacterium Methanococcus vannielii. The 3' poly(A) tracts in poly(A)+ RNA molecules are approximately twice as long (average length of 20 nucleotides) in H. halobium as in M. vannielii. In both archaebacterial species, poly(A)+ RNAs are unstable.
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