Open AccessLysogenic conversion of staphylococcal lipase is caused by insertion of the bacteriophage L54a genome into the lipase structural gene
Author(s) -
Chia-Yen Lee,
John J. Iandolo
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.166.2.385-391.1986
Subject(s) - lysogenic cycle , biology , lipase , nucleic acid sequence , bacteriophage , gene , structural gene , nucleotide , dna , staphylococcus aureus , genetics , microbiology and biotechnology , biochemistry , escherichia coli , enzyme , bacteria
Staphylococcus aureus PS54 manifests no lipase (geh) activity. This is due to the insertion of bacteriophage L54a DNA into the geh structural gene. The nucleotide sequence of this 2,968-base-pair DNA fragment was determined. Lipase deduced from the nucleotide sequence is a polypeptide of 690 amino acids which extends from nucleotide 706 to 2776.