Open AccessPhysical and functional map of supervirulent Agrobacterium tumefaciens tumor-inducing plasmid pTiBo542
Author(s) -
Toshihiko Komari,
Walter Halperin,
Eugene W. Nester
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.166.1.88-94.1986
Subject(s) - agrobacterium tumefaciens , biology , plasmid , virulence , gene , genetics , ti plasmid , t dna binary system , clone (java method) , agrobacterium , bamhi , restriction map , transfer dna , vector (molecular biology) , transformation (genetics) , recombinant dna
Agrobacterium tumefaciens strains carrying pTiBo542 induce large, fast-appearing tumors and have an unusually wide host range. A clone bank was made from this 250-kilobase plasmid in a wide-host-range vector, and restriction maps were determined for BamHI and SalI. The virulence genes, transferred DNA genes, plasmid incompatibility region, and a region that inhibits growth of certain A. tumefaciens strains were localized. The six virulence genes and two tms genes were highly homologous to the genes of pTiA6, but the tmr gene was not. Mutations in each of the six vir loci of pTiA6 were complemented by clones from the vir region of pTiBo542.