Open AccessAnalysis of developmentally defective chemical signaling mutants of Polysphondylium violaceum
Author(s) -
Catherine F. Ellsaesser,
Peter Kühn,
Michael H. Hanna
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.165.2.647-649.1986
Subject(s) - mutant , complementation , biology , mutagenesis , genetics , mutation , protein fragment complementation assay , microbiology and biotechnology , gene
Six aggregation-defective mutants of Polysphondylium violaceum dependent on external addition of the pheromone D factor for aggregation were isolated after nitrosoguanidine mutagenesis. With a screening technique based on synergistic development, D-factor-dependent mutants can be separated from other kinds of aggregateless mutants. Genetic complementation analyses of the newly isolated mutants showed them to be mutant at the aggA locus. Individual mutants exhibited different sensitivities to D factor(s), responding maximally over a 300-fold range of concentrations.