Open AccessSpecialized transduction of Pseudomonas aeruginosa PAO by bacteriophage D3
Author(s) -
Margaret M. Cavenagh,
Robert V. Miller
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.165.2.448-452.1986
Subject(s) - lysogen , biology , prophage , bacteriophage , transduction (biophysics) , complementation , temperateness , lysogenic cycle , allele , pseudomonas aeruginosa , superinfection , genetics , microbiology and biotechnology , escherichia coli , virus , gene , bacteria , biochemistry , phenotype
D3, a temperate bacteriophage of Pseudomonas aeruginosa PAO, was found to specifically transduce the alleles met-49 and met-117. Induction of established lysogens with UV light was necessary for the production of transducing lysates. Transduced cells were immune to superinfection by phage D3 and could give rise to high-frequency transducing lysates. Cotransduction of these two alleles could not be demonstrated. met-117 was mapped to 26 min on the PAO genetic map. Complementation studies using the generalized transducing phage F116L indicated that met-49 is an allele of met-9011 which maps at 55 min. The integrated D3 prophage was shown to be coinherited with met-117 and with met-49.