Open AccessIntegration of the bacteriophage HP1c1 genome into the Haemophilus influenzae Rd chromosome in the lysogenic state
Author(s) -
Alan S. Waldman,
Wayne P. Fitzmaurice,
John J. Scocca
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.165.1.297-300.1986
Subject(s) - lysogenic cycle , lysogen , biology , bacteriophage , phagemid , temperateness , genetics , genome , dna , prophage , gene , escherichia coli
Restriction fragments hybridizing to phage HP1c1 DNA were identified in digests of DNA from lysogenic strains of Haemophilus influenzae. The results showed that the cohesive ends of the mature phage DNA were joined in lysogens and that the phage genome was covalently linked to the host DNA, indicating that lysogeny involves recombination between specific sites on the phage and host chromosomes. The site on the phage chromosome at which this recombination occurred was between 110 and 750 base pairs of the left end on the mature phage genome.