Open AccessThiamine transport in Saccharomyces cerevisiae protoplasts
Author(s) -
Hiroshi Nishimura,
Kenji Sempuku,
Akio Iwashima
Publication year - 1982
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.150.2.960-962.1982
Subject(s) - thiamine , saccharomyces cerevisiae , thiamine pyrophosphate , protoplast , biology , yeast , biochemistry , periplasmic space , saccharomyces , organelle , enzyme , cofactor , gene , escherichia coli
Thiamine was found to be accumulated in protoplasts of Saccharomyces cerevisiae in the same manner as in intact cells, suggesting that a soluble thiamine-binding protein in periplasm may not be an essential component of the thiamine transport system of S. cerevisiae. It was also found that thiamine pyrophosphate cannot be taken up by yeast protoplasts.
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