Open AccessExtracellular exonuclease as a stage 0 biochemical marker in Bacillus subtilis sporulation
Author(s) -
William F. Burke,
Barbara S. Slinker
Publication year - 1982
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.149.2.768-770.1982
Subject(s) - biology , bacillus subtilis , nuclease , exonuclease , proteases , extracellular , spore , enzyme , biochemistry , microbiology and biotechnology , dna , genetics , bacteria , dna polymerase
Calcium-dependent exonuclease activity was produced by sporulating cells of Bacillus subtilis 168. Nuclease activity was released into the culture medium at approximately the same time as sporulation proteases, and production of these enzymes was tied to DNA replication. Results suggest that nuclease production is a function of the spo0H locus.
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