Open AccessUse of Transposons in Cloning Poorly Selectable Genes of Escherichia coli : Cloning of uvrA and Adjacent Genes
Author(s) -
Jourica A. Brandsma,
Cees A. van Sluis,
Pieter van de Putte
Publication year - 1981
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.147.2.682-684.1981
Subject(s) - biology , transposable element , selectable marker , genetics , cloning (programming) , gene , sleeping beauty transposon system , escherichia coli , molecular cloning , plasmid , mutant , peptide sequence , programming language , computer science
A transposon was introduced close to a poorly selectable gene. This gene could be cloned by using selection for the antibiotic resistance marker of the transposon.
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