Physical map of the Salmonella typhimurium histidine transport operon: correlation with the genetic map | Zendy

Feroza Ardeshir | Zendy; Christopher F. Higgins | Zendy; G F Ames | Zendy

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Physical map of the Salmonella typhimurium histidine transport operon: correlation with the genetic map

Author(s) -

Feroza Ardeshir,

Christopher F. Higgins,

G F Ames

Publication year - 1981

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.147.2.401-409.1981

Subject(s) - operon , biology , restriction map , ecori , plasmid , dna , gal operon , salmonella , genetics , histidine , l arabinose operon , microbiology and biotechnology , gene , trp operon , escherichia coli , bacteria , amino acid

A detailed restriction map of a 12.4-kilobase EcoRI fragment of Salmonella typhimurium deoxyribonucleic acid (DNA) containing the entire histidine transport operon and the argT gene is presented. Subclones of specific regions of the transport operon of S. typhimurium were constructed in plasmid vectors. An accurate correlation between the restriction map and the location of genetically defined deletions was obtained by hybridizing restriction digests of chromosomal DNA from strains carrying each deletion with cloned transport operon DNA as a probe. These data were used to position the histidine transport genes on the cloned 12.4-kilobase fragment of DNA.

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