Open AccessOrganization and regulation of the ilvGEDA operon in Salmonella typhimurium LT2
Author(s) -
Christina Berg,
Karen Joy Shaw
Publication year - 1981
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.145.2.984-989.1981
Subject(s) - operon , cistron , biology , tn10 , transposable element , genetics , mutant , l arabinose operon , gal operon , trp operon , gene , mutation , lac operon , isoleucine , escherichia coli , amino acid , leucine
A total of 102 isoleucine- and isoleucine-valine-requiring (ilv) mutants induced by insertion of the transposable element Tn10 have been classified to cistron by growth requirement, cross-feeding behavior, and enzyme assays. The mutations are in a polycistronic operon transcribed in the order ilvGEDA and in a monocistronic operon ilvC. Analysis of distal gene expression in these polar insertion mutants revealed the existence of two constitutive interval promoters, one preceding ilvE and the other preceding ilvD.
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