Open AccessCharacterization of a site-specific restriction endonuclease from Streptomyces aureofaciens
Author(s) -
Josef Timko,
Arnold H. Horwitz,
J. Zelinka,
Gary Wilcox
Publication year - 1981
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.145.2.873-877.1981
Subject(s) - biology , exonuclease , streptomyces aureofaciens , restriction enzyme , exonuclease iii , microbiology and biotechnology , endonuclease , dna , streptomyces , pbr322 , nucleic acid sequence , lambda phage , genetics , biochemistry , bacteriophage , escherichia coli , gene , dna polymerase , bacteria , plasmid
A new type II sequence-specific restriction endonuclease, SauI, was isolated from Streptomyces aureofaciens IKA18/4. The purified enzyme was free of contaminating exonuclease and phosphatase activities. SauI cleaved lambda DNA at two sites, but did not cleave pBR322, simian virus 40, or phi X174 DNA. SauI recognized the septanucleotide sequence 5'-CCTNAGG-3' and cleaved at the position indicated by the arrow, producing a trinucleotide 5'-terminal extension.
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