Deoxyribonucleic acid polymerase II activity in an Escherichia coli mutator strain | Zendy

Carolyn L. Smith | Zendy; H. Shizuya | Zendy; R E Moses | Zendy

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Deoxyribonucleic acid polymerase II activity in an Escherichia coli mutator strain

Author(s) -

Carolyn L. Smith,

H. Shizuya,

R E Moses

Publication year - 1976

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.125.1.191-196.1976

Subject(s) - biology , dna polymerase , escherichia coli , dna polymerase i , polymerase , microbiology and biotechnology , dna polymerase ii , dna , mutant , dna clamp , gene , polymerase chain reaction , genetics , reverse transcriptase

The polB gene encoding deoxyribonucleic acid (DNA) polymerase II has been located close to a mutator gene, mutT1, in Escherichia coli. We find the DNA polymerase II prepared from mutT1, strains to be normal in reaction requirements, heat stability, and ability to remove mismatched bases at termini. Recombinants formed from a mutant defective in DNA polymerase II (polB100) and mutT1 are deficient in polymerase II and have the same mutator phenotype as mutT1. Our linkage analysis indicates that mutT1 and polB100 are not isoallelic.

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