Selection of Respiratory Mutants of Neurospora crassa | Zendy

David L. Edwards | Zendy; Fabia Kwiecinski | Zendy; Jonathan Horstmann | Zendy

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Selection of Respiratory Mutants of Neurospora crassa

Author(s) -

David L. Edwards,

Fabia Kwiecinski,

Jonathan Horstmann

Publication year - 1973

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.114.1.164-168.1973

Subject(s) - biology , respiration , neurospora crassa , mutant , tetrazolium chloride , cyanide , antimycin a , mitochondrion , biochemistry , inositol , cellular respiration , botany , chemistry , medicine , inorganic chemistry , receptor , ischemia , cardiology , gene

A method is described that permits the isolation of mutants that are defective in mitochondrial respiration. The techniques of inositol-less death and overlay with 2,3,5-triphenyl-2H-tetrazolium chloride are utilized to select for mutant colonies. Colonies that survive inositol-less death and fail to reduce the tetrazolium dye are then tested polarographically for cyanide-sensitive respiration. A preliminary characterization of three mutants obtained by this method is presented. The mutants have been characterized by their cyanide-sensitive respiration rate, growth rate, the state III respiration rate of isolated mitochondria, inhibition of the respiration of isolated mitochondria by cyanide and antimycin A, and cytochrome spectra. All of the mutants described differ from the parent strain in some of these aspects.

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