Open AccessCharacterization of Components of the Staphylococcus aureus mRNA Degradosome Holoenzyme-Like Complex
Author(s) -
Christelle M. Roux,
Jonathon P. DeMuth,
Paul M. Dunman
Publication year - 2011
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.05485-11
Subject(s) - degradosome , polynucleotide phosphorylase , rnase p , biology , rna helicase a , rnase mrp , rnase h , staphylococcus aureus , exosome complex , rna , helicase , purine nucleoside phosphorylase , genetics , biochemistry , enzyme , bacteria , gene , purine
Bacterial two-hybrid analysis identified theStaphylococcus aureus RNA degradosome-like complex to include RNase J1, RNase J2, RNase Y, polynucleotide phosphorylase (PNPase), enolase, phosphofructokinase, and a DEAD box RNA helicase. Results also revealed that the recently recognized RNase RnpA interacts with theS. aureus degradosome and that this interaction is conserved in other Gram-positive organisms.