Open AccessTranscriptional Regulation of Fatty Acid Biosynthesis in Lactococcus lactis
Author(s) -
Tom H. Eckhardt,
Dorota Skotnicka,
Jan Kok,
Oscar P. Kuipers
Publication year - 2012
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.02043-12
Subject(s) - biology , lactococcus lactis , regulator , repressor , transcriptional regulation , gene , electrophoretic mobility shift assay , biochemistry , regulation of gene expression , transcription (linguistics) , transcriptome , transcription factor , genetics , gene expression , bacteria , lactic acid , linguistics , philosophy
Here we study the influence of the putative fatty acid biosynthesis (FAB) regulator FabT (originally called RmaG [Llmg_1788]) on gene transcription in Lactococcus lactis MG1363. A strain with a knockout mutation of the putative regulator was constructed, and its transcriptome was compared to that of the wild-type strain. Almost all FAB genes were significantly upregulated in the knockout. Using electrophoretic mobility shift assays (EMSAs) and DNase I footprinting, the binding motif of the regulator and the binding locations in the genome were characterized. Fatty acid composition analysis revealed that a strain lacking FabT contained significantly more saturated acyl chains in its phospholipids. This observation demonstrates that the vital pathway of FAB in L. lactis is regulated by the repressor FabT.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom