Microarray Analysis of Transposon Insertion Mutations in Bacillus anthracis : Global Identification of Genes Required for Sporulation and Germination | Zendy

William Alan Day | Zendy; Suzanne L. Rasmussen | Zendy; Beth M. Carpenter | Zendy; Scott N. Peterson | Zendy; Arthur M. Friedlander | Zendy

Open Access

Microarray Analysis of Transposon Insertion Mutations in Bacillus anthracis : Global Identification of Genes Required for Sporulation and Germination

Author(s) -

William Alan Day,

Suzanne L. Rasmussen,

Beth M. Carpenter,

Scott N. Peterson,

Arthur M. Friedlander

Publication year - 2007

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.01860-06

Subject(s) - biology , transposable element , bacillus anthracis , gene , genetics , genome , phenotype , spore germination , spore , microbiology and biotechnology , bacteria

A transposon site hybridization (TraSH) assay was developed for functional analysis of the Bacillus anthracis genome using a mini-Tn10 transposon which permitted analysis of 82% of this pathogen's genes. The system, used to identify genes required for generation of infectious anthrax spores, spore germination, and optimal growth on rich medium, was predictive of the contributions of two conserved hypothetical genes for the phenotypes examined.

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