Open AccessPolarity in Archaeal Operon Transcription in Thermococcus kodakaraensis
Author(s) -
Thomas J. Santangelo,
Ľubomíra Čuboňová,
Rie Matsumi,
Haruyuki Atomi,
Tadayuki Imanaka,
John N. Reeve
Publication year - 2008
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.01811-07
Subject(s) - thermococcus , operon , biology , gene , genetics , reporter gene , gene expression , archaea , escherichia coli
An in vivo archaeal gene reporter system has been established based on TK1761, a gene that encodes a nonessential β-glycosidase inThermococcus kodakaraensis . Following the introduction of nonsense codons into promoter-proximal genes, polarity in operon expression in this archaeon has been established by both microarray hybridization assays and a reporter gene expression system.