Polarity in Archaeal Operon Transcription in Thermococcus kodakaraensis | Zendy

Thomas J. Santangelo | Zendy; L’ubomı́ra Čuboňová | Zendy; Rie Matsumi | Zendy; Haruyuki Atomi | Zendy; Tadayuki Imanaka | Zendy; John N. Reeve | Zendy

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Polarity in Archaeal Operon Transcription in Thermococcus kodakaraensis

Author(s) -

Thomas J. Santangelo,

L’ubomı́ra Čuboňová,

Rie Matsumi,

Haruyuki Atomi,

Tadayuki Imanaka,

John N. Reeve

Publication year - 2008

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.01811-07

Subject(s) - thermococcus , operon , biology , gene , genetics , reporter gene , gene expression , archaea , escherichia coli

An in vivo archaeal gene reporter system has been established based on TK1761, a gene that encodes a nonessential β-glycosidase inThermococcus kodakaraensis . Following the introduction of nonsense codons into promoter-proximal genes, polarity in operon expression in this archaeon has been established by both microarray hybridization assays and a reporter gene expression system.

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