Open AccessAn RNA Hairpin Sequesters the Ribosome Binding Site of the Homing Endonuclease mobE Gene
Author(s) -
Ewan A. Gibb,
David R. Edgell
Publication year - 2009
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.01751-08
Subject(s) - biology , homing endonuclease , ribosome , endonuclease , binding site , rna , a site , homing (biology) , ribosomal binding site , genetics , gene , transfer rna , rna binding protein , microbiology and biotechnology , ecology
Previous transcript mapping of the bacteriophage Aeh1nrd operon revealed a predicted RNA hairpin upstream of the homing endonucleasemobE gene. We enzymatically mapped the hairpin, showing that themobE ribosome binding site is sequestered. Cloning of the hairpin upstream oflacZ resulted in reduced β-galactosidase activity, consistent with translational regulation.