Open AccessAnalysis of Protein Expression Regulated by the Helicobacter pylori ArsRS Two-Component Signal Transduction System
Author(s) -
John T. Loh,
Shobhana Satyendra Gupta,
David Friedman,
Andrzej M. Krezel,
Timothy L. Cover
Publication year - 2010
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.01703-08
Subject(s) - regulon , biology , mutant , two component regulatory system , signal transduction , biochemistry , response regulator , thioredoxin , gene expression , quorum sensing , helicobacter pylori , gene , microbiology and biotechnology , genetics , virulence
Previous studies have shown that theHelicobacter pylori ArsRS two-component signal transduction system contributes to acid-responsive gene expression. To identify additional members of the ArsRS regulon and further investigate the regulatory role of the ArsRS system, we analyzed protein expression in wild-type andarsS null mutant strains. Numerous proteins were differentially expressed in anarsS mutant strain compared to a wild-type strain when the bacteria were cultured at pH 5.0 and also when they were cultured at pH 7.0. Genes encoding 14 of these proteins were directly regulated by the ArsRS system, based on observed binding of ArsR to the relevant promoter regions. The ArsRS-regulated proteins identified in this study contribute to acid resistance (urease and amidase), acetone metabolism (acetone carboxylase), resistance to oxidative stress (thioredoxin reductase), quorum sensing (Pfs), and several other functions. These results provide further definition of the ArsRS regulon and underscore the importance of the ArsRS system in regulating expression ofH. pylori proteins during bacterial growth at both neutral pH and acidic pH.