YjgF Is Required for Isoleucine Biosynthesis whenSalmonella entericaIs Grown on Pyruvate Medium | Zendy

Melissa R. Christopherson | Zendy; George E. Schmitz | Zendy; Diana M. Downs | Zendy

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YjgF Is Required for Isoleucine Biosynthesis whenSalmonella entericaIs Grown on Pyruvate Medium

Author(s) -

Melissa R. Christopherson,

George E. Schmitz,

Diana M. Downs

Publication year - 2008

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.01700-07

Subject(s) - isoleucine , salmonella enterica , biology , mutant , serine , biochemistry , biosynthesis , salmonella , microbiology and biotechnology , bacteria , escherichia coli , amino acid , leucine , genetics , enzyme , gene

The YjgF/YER057c/UK114 family of proteins is conserved across the three domains of life, yet no biochemical function has been clearly defined for any member of this family. InSalmonella enterica , a deletion ofyjgF results in a requirement for isoleucine when the mutant strain is grown in glucose-serine or pyruvate medium. Feedback inhibition of IlvA is required for the curative effect of isoleucine on glucose-serine medium. On pyruvate medium,yjgF mutants are unable to synthesize enough isoleucine for growth. From this study, we conclude that the isoleucine requirement of ayjgF mutant on pyruvate is a consequence of the decreased transaminase B (IlvE) activity that has previously been characterized in these mutants.

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