Open AccessCritical Role of a Single Position in the −35 Element for Promoter Recognition by Mycobacterium tuberculosis SigE and SigH
Author(s) -
Taeksun Song,
Suk-Won Song,
Sahadevan Raman,
Mauricio Anaya,
Robert N. Husson
Publication year - 2008
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.01642-07
Subject(s) - promoter , biology , mycobacterium tuberculosis , primer extension , transcription (linguistics) , response element , genetics , tuberculosis , gene , base sequence , gene expression , medicine , linguistics , philosophy , pathology
Mycobacterial SigE and SigH both initiate transcription from thesigB promoter, suggesting that they recognize similar sequences. Through mutational and primer extension analyses, we determined that SigE and SigH recognize nearly identical promoters, with differences at the 3′ end of the −35 element distinguishing between SigE- and SigH-dependent promoters.