Colony Spreading in Staphylococcus aureus

Wild-typeStaphylococcus aureus rapidly expands on the surface of soft agar plates. The rates of expansion and the shapes of the resultant giant colonies were distinct for different strains of laboratory stocks and clinical isolates. The colony spreading abilities did not correlate with the biofilm-forming abilities in these strains. Insertional disruption of thedltABCD operon, which functions at the step ofd -alanine addition to teichoic acids, and of thetagO gene, which is responsible for the synthesis of wall teichoic acids, decreased the colony spreading ability. The results indicate that wall teichoic acids andd -alanylation of teichoic acids are required for colony spreading.