Open AccessSulfolobus Replication Factor C Stimulates the Activity of DNA Polymerase B1
Author(s) -
Xuanxuan Xing,
Likui Zhang,
Li Guo,
Qunxin She,
Li Huang
Publication year - 2014
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.01552-14
Subject(s) - biology , dna polymerase , dna clamp , dna polymerase ii , dna replication , dna polymerase delta , dna polymerase i , primer (cosmetics) , replication factor c , proliferating cell nuclear antigen , polymerase , eukaryotic dna replication , primase , microbiology and biotechnology , dna , genetics , gene , chemistry , reverse transcriptase , polymerase chain reaction , organic chemistry
Replication factor C (RFC) is known to function in loading proliferating cell nuclear antigen (PCNA) onto primed DNA, allowing PCNA to tether DNA polymerase for highly processive DNA synthesis in eukaryotic and archaeal replication. In this report, we show that an RFC complex from the hyperthermophilic archaea of the genus Sulfolobus physically interacts with DNA polymerase B1 (PolB1) and enhances both the polymerase and 3'-5' exonuclease activities of PolB1 in an ATP-independent manner. Stimulation of the PolB1 activity by RFC is independent of the ability of RFC to bind DNA but is consistent with the ability of RFC to facilitate DNA binding by PolB1 through protein-protein interaction. These results suggest that Sulfolobus RFC may play a role in recruiting DNA polymerase for efficient primer extension, in addition to clamp loading, during DNA replication.
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