Open AccessTn 5 Synaptic Complex Formation: Role of Transposase Residue W450
Author(s) -
Richard J. Gradman,
William S. Reznikoff
Publication year - 2007
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.01488-07
Subject(s) - biology , transposase , synapsis , residue (chemistry) , genetics , amino acid , biophysics , biochemistry , transposable element , mutant , gene , recombination
A series of Tn5 transposases (Tnp's) with mutations at the conserved amino acid position W450, which was structurally predicted to be important for synapsis, have been generated and characterized. This study demonstrates that W450 is involved in hydrophobic (and possibly aromatic) contacts within the Tnp monomer that negatively regulate synaptic complex formation.
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