Open AccessThe Regulatory C-Terminal Domain of Subunit ε of F o F 1 ATP Synthase Is Dispensable for Growth and Survival of Escherichia coli
Author(s) -
Noriyuki Taniguchi,
Toshiharu Suzuki,
Michael Berney,
Masasuke Yoshida,
Gregory M. Cook
Publication year - 2011
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.01422-10
Subject(s) - biology , escherichia coli , atp synthase , protein subunit , terminal (telecommunication) , escherichia coli proteins , domain (mathematical analysis) , biochemistry , microbiology and biotechnology , genetics , gene , telecommunications , mathematical analysis , mathematics , computer science
The C-terminal domain of subunit ε of the bacterial Fo F1 ATP synthase is reported to be an intrinsic inhibitor of ATP synthesis/hydrolysis activityin vitro , preventing wasteful hydrolysis of ATP under low-energy conditions. Mutants defective in this regulatory domain exhibited no significant difference in growth rate, molar growth yield, membrane potential, or intracellular ATP concentration under a wide range of growth conditions and stressors compared to wild-type cells, suggesting this inhibitory domain is dispensable for growth and survival ofEscherichia coli .