Open AccessEscherichia coli Physiology in Luria-Bertani Broth
Author(s) -
Guennadi Sezonov,
Danièle JoseleauPetit,
Richard D’Ari
Publication year - 2007
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.01368-07
Subject(s) - biology , escherichia coli , microbiology and biotechnology , enterobacteriaceae , bacteriology , bacteria , biochemistry , genetics , gene
Luria-Bertani broth supports Escherichia coli growth to an optical density at 600 nm (OD(600)) of 7. Surprisingly, however, steady-state growth ceases at an OD(600) of 0.3, when the growth rate slows down and cell mass decreases. Growth stops for lack of a utilizable carbon source. The carbon sources for E. coli in Luria-Bertani broth are catabolizable amino acids, not sugars.
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