Open AccessThe Salmonella SPI1 Type Three Secretion System Responds to Periplasmic Disulfide Bond Status via the Flagellar Apparatus and the RcsCDB System
Author(s) -
Dongxia Lin,
Christopher V. Rao,
James M. Slauch
Publication year - 2008
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.01323-07
Subject(s) - periplasmic space , dsba , biology , effector , microbiology and biotechnology , salmonella enterica , pathogenicity island , type three secretion system , secretion , transcription factor , virulence , salmonella , gene , genetics , biochemistry , escherichia coli , bacteria
Upon contact with intestinal epithelial cells,Salmonella enterica serovar Typhimurium injects a set of effector proteins into the host cell cytoplasm via theSalmonella pathogenicity island 1 (SPI1) type III secretion system (T3SS) to induce inflammatory diarrhea and bacterial uptake. The master SPI1 regulatory genehilA is controlled directly by three AraC-like regulators: HilD, HilC, and RtsA. Previous work suggested a role for DsbA, a periplasmic disulfide bond oxidase, in SPI1 T3SS function. RtsA directly activatesdsbA , and deletion ofdsbA leads to loss of SPI1-dependent secretion. We have studied thedsbA phenotypes by monitoring expression of SPI1 regulatory, structural, and effector genes. Here we present evidence that loss of DsbA independently affects SPI1 regulation and SPI1 function. ThedsbA -mediated feedback inhibition of SPI1 transcription is not due to defects in the SPI1 T3SS apparatus. Rather, the transcriptional response is dependent on both the flagellar protein FliZ and the RcsCDB system, which also affectsfliZ transcription. Thus, the status of disulfide bonds in the periplasm affects expression of the SPI1 system indirectly via the flagellar apparatus. RcsCDB can also affect SPI1 independently of FliZ. All regulation is through HilD, consistent with our current model for SPI1 regulation.