Function of Ech Hydrogenase in Ferredoxin-Dependent, Membrane-Bound Electron Transport in Methanosarcina mazei | Zendy

Cornelia U. Welte | Zendy; Verena Kallnik | Zendy; Marcel Grapp | Zendy; Güneş Bender | Zendy; Steve W. Ragsdale | Zendy; Uwe Deppenmeier | Zendy

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Function of Ech Hydrogenase in Ferredoxin-Dependent, Membrane-Bound Electron Transport in Methanosarcina mazei

Author(s) -

Cornelia U. Welte,

Verena Kallnik,

Marcel Grapp,

Güneş Bender,

Steve W. Ragsdale,

Uwe Deppenmeier

Publication year - 2009

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.01307-09

Subject(s) - ferredoxin , methanogenesis , hydrogenase , methanosarcina , methanosarcina barkeri , electron transport chain , biology , ferredoxin—nadp(+) reductase , biochemistry , electron transfer , reductase , biophysics , chemistry , enzyme , photochemistry , methane , ecology

Reduced ferredoxin is an intermediate in the methylotrophic and aceticlastic pathway of methanogenesis and donates electrons to membrane-integral proteins, which transfer electrons to the heterodisulfide reductase. A ferredoxin interaction has been observed previously for the Ech hydrogenase. Here we present a detailed analysis of aMethanosarcina mazei Δech mutant which shows decreased ferredoxin-dependent membrane-bound electron transport activity, a lower growth rate, and faster substrate consumption. Evidence is presented that a second protein whose identity is unknown oxidizes reduced ferredoxin, indicating an involvement in methanogenesis from methylated C1 compounds.

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