Reconstitution of the Biochemical Activities of the AttJ Repressor and the AttK, AttL, and AttM Catabolic Enzymes of Agrobacterium tumefaciens | Zendy

Yunrong Chai | Zendy; Ching Sung Tsai | Zendy; Hongbaek Cho | Zendy; Stephen C. Winans | Zendy

Open Access

Reconstitution of the Biochemical Activities of the AttJ Repressor and the AttK, AttL, and AttM Catabolic Enzymes of Agrobacterium tumefaciens

Author(s) -

Yunrong Chai,

Ching Sung Tsai,

Hongbaek Cho,

Stephen C. Winans

Publication year - 2007

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.01274-06

Subject(s) - biology , agrobacterium tumefaciens , catabolism , repressor , enzyme , agrobacterium , biochemistry , transformation (genetics) , gene , transcription factor

The attKLM operon encodes a lactonase (AttM) that hydrolyzes acylhomoserine lactone autoinducers, as well as two putative dehydrogenases (AttK and AttL). Here we show that AttK, AttL, and AttM collectively covert gamma-butyrolactone to succinate. Two metabolic intermediates, gamma-hydroxybutyrate and succinic semialdehyde, inactivated the AttJ repressor in vitro and induced attKLM transcription in vivo.

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