Open AccessCharacterization of the CtsR Stress Response Regulon in Lactobacillus plantarum
Author(s) -
Daniela Fiocco,
Vittorio Capozzi,
Michael Collins,
Anna Gallone,
Pascal Hols,
Jean Guzzo,
Stéphanie Weidmann,
Aurélie Rieu,
Tarek Msadek,
Giuseppe Spano
Publication year - 2010
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.01122-09
Subject(s) - regulon , biology , lactobacillus plantarum , operon , genetics , gene , mutant , promoter , gene expression , bacteria , lactic acid
Lactobacillus plantarum ctsR was characterized. ctsR was found to be cotranscribed with clpC and induced in response to various abiotic stresses. ctsR deletion conferred a heat-sensitive phenotype with peculiar cell morphological features. The transcriptional pattern of putative CtsR regulon genes was examined in the Delta ctsR mutant. Direct CtsR-dependent regulation was demonstrated by DNA-binding assays using recombinant CtsR and the promoters of the ctsR-clpC operon and hsp1.