Open AccessMaturation of the Translation Inhibitor Microcin C
Author(s) -
Anastasia Metlitskaya,
Teymur Kazakov,
Gaston Vondenhoff,
М. А. Новикова,
Alexander S. Shashkov,
Timofei S. Zatsepin,
Ekaterina Semenova,
Наталья Владимировна Зайцева,
Vasily Ramensky,
Arthur Van Aerschot,
Konstantin Severinov
Publication year - 2009
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.00999-08
Subject(s) - biology , biochemistry , translation (biology) , biosynthesis , gene , residue (chemistry) , bacteria , protein biosynthesis , genetics , messenger rna
Microcin C (McC), an inhibitor of the growth of enteric bacteria, consists of a heptapeptide with a modified AMP residue attached to the backbone of the C-terminal aspartate through an N-acyl phosphamidate bond. Here we identify maturation intermediates produced by cells lacking individual mcc McC biosynthesis genes. We show that the products of the mccD and mccE genes are required for attachment of a 3-aminopropyl group to the phosphate of McC and that this group increases the potency of inhibition of the McC target, aspartyl-tRNA synthetase.