Open AccessPolymerization of the Actin-Like Protein MamK, Which Is Associated with Magnetosomes
Author(s) -
Azuma Taoka,
Ryuji Asada,
LongFei Wu,
Yoshihiro Fukumori
Publication year - 2007
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.00899-07
Subject(s) - biology , recombinant dna , actin , escherichia coli , immunostaining , magnetosome , microbiology and biotechnology , actin binding protein , in vitro , polymerization , biochemistry , bacteria , cytoskeleton , immunology , cell , actin cytoskeleton , immunohistochemistry , polymer , gene , genetics , chemistry , organic chemistry
The recombinant actin-like protein MamK was purified fromEscherichia coli and used as an antigen to generate the anti-MamK antibody. Immunostaining studies showed a linear distribution of MamK inMagnetospirillum magnetotacticum cells and of MamK in association with magnetosomes. Moreover, we demonstrated that MamK polymerizes into filamentous bundles in vitro.